Figure 2.

The −953 region of the cyclin D1 promoter is required for activation by small t. (A) A series of 5′ promoter deletions of the cyclin D1 promoter were transfected into JEG-3 cells with the small t expression plasmid. The data are the mean fold induction ± SEM by pw2t determined by comparison with cells transfected with the “cassette” dl888. Data is for N separate transfections as indicated in parenthesis. (B) The cyclin D1(AP-1)TKLUC reporter was transfected with small t into JEG-3 cells. Data are normalized for the effect of the pw2t vector (shown as 1). Comparison was made with the effect of small t on the synthetic reporters, CD1(E2F)TKLUC, CD1(CRE)TKLUC (encoding the sequences resembling a putative CRE binding site at −57), and the synthetic AP-1 site reporter p₃TPLUX. The data are shown expressed as mean ± SEM.