Figure 2.
FL-SA-Ro selectively labeled the cleavage furrows of intact CHO cells during cytokinesis. Cells at prometaphase (A), anaphase (B), late telophase (C), and G1 phase (D) were incubated with FL-SA-Ro for 30 min at 4°C, then washed and photographed. Phase contrast micrographs, FL-SA-Ro staining and 4′-6-diamino-2-phenylindole (DAPI) fluorescence of the same specimens, respectively, are shown. (E) Late telophase cells were double-labeled with FL-SA-Ro and TRITC-phalloidin. Note that strong fluorescence was observed only at the cleavage furrow during cytokinesis; indicated by arrows in C. (Bar = 10 mm.)