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Immunoblot analysis of reducing 12% polyacrylamide gels of lysates from monolayers of HA-expressing CV 1 cells following incubation with (+) or without (-) trypsin, to show cell surface expression of HA. Lane pairs: A, wild type; B, R542E; C, E812G; D, H171Q,G2181E; E, H171Q,K582I; F, R542E,K582I; G, Q472R,K582I; H, E812G,K582I; I, G2181E,K582I; J, H171Q,D1122G; K, wild type; L, D1122G,K582I; M, G2181E; N, K582I; O, D1122G; P, H171Q; and Q, Q472R.
