Table I.

Protein spot identifications with 3-fold change or more in relative expression at 120 versus 72 hpf

h., heavy; l., light; mitochondr., mitochondrial; Eukar. transl. init. fact., eukaryotic translation initiation factor; Het., heterogeneous; contain., containing; prot., protein; APEX, apurinic/apyrimidinic endonuclease.

Spota	Accessionb	Protein name	Scorec	Molecular weight	pI	ID Spec.d	Pep. count (n)e	Unmtch. (n)f	Cov.g	-Fold changeh
									%
1699	IPI00503469	Actin	152	41947	5.22	590	11	61	40	24.1
2270	IPI00551966	Actin, α1, skeletal muscle	90	41955	5.23	607	8	54	31	10.8
2124	IPI00482295	Actin, cytoplasmic 1	74	41739	5.3	580	6	71	18	10.3
1709	IPI00503469	Actin	101	41947	5.22	601	7	54	23	10
2071	IPI00503469	Actin	134	41947	5.22	593	12	57	36	8.5
2068	IPI00503469	Actin	135	41947	5.22	587	10	56	35	7
1752	IPI00503469	Actin	115	41947	5.22	571	10	64	38	5
2001	IPI00503469	Actin	103	41947	5.22	606	9	53	32	4.7
2315	IPI00503469	Actin	90	41947	5.22	568	7	64	21	4.6
2298	IPI00503469	Actin	85	41947	5.22	608	7	52	21	4.4
2096	IPI00503469	Actin	113	41947	5.22	564	7	69	27	4.2
1466	IPI00503469	Actin	219	41947	5.22	569	12	60	41	3.3
2049	IPI00503469	Actin	85	41947	5.22	577	9	66	32	3.1
1510	IPI00551966	Actin, α1, skeletal muscle	103	41955	5.23	63	8	63	30	−4.4
1392	IPI00503469	Actin	89	41947	5.22	613	9	58	33	−13.7
1379	IPI00503469	Actin	102	41947	5.22	611	9	50	29	−16.8
1393	IPI00503469	Actin	222	41947	5.22	636	12	49	44	−19.2
1919	IPI00483287	Fast skeletal myosin h. chain 3	94	51813	5.49	583	13	50	29	27.3
1713	IPI00497758	Fast myosin heavy chain 4	74	222067	5.54	566	12	40	9	14.5
1861	IPI00483287	Fast skeletal myosin h. chain 3	91	51813	5.49	572	13	63	31	11.6
1834	IPI00483287	Fast skeletal myosin h. chain 3	88	51813	5.49	603	14	58	31	7
1766	IPI00497758	Fast myosin heavy chain 4	76	222067	5.54	582	20	55	11	6.4
1803	IPI00509014	α-Tropomyosin	226	32720	4.7	592	18	58	47	5.3
1822	IPI00497758	Fast myosin heavy chain 4	65	222067	5.54	610	18	57	12	3.7
1605	IPI00509014	α-Tropomyosin	241	32720	4.7	117	20	51	49	3.3
1338	IPI00483287	Fast skeletal myosin h. chain 3	79	51813	5.49	638	13	58	29	−3.2
1519	IPI00497758	Fast myosin heavy chain 4	165	222067	5.54	674	19	23	10	−3.3
1975	IPI00499941	Fast skeletal myosin l. chain 1a	138	20918	4.63	668	10	54	61	−3.4
1467	IPI00497758	Fast myosin heavy chain 4	139	222067	5.54	657	21	33	10	−3.4
1495	IPI00483287	Fast skeletal myosin h. chain 3	105	51813	5.49	666	14	41	30	−3.7
1595	IPI00483287	Fast skeletal myosin h. chain 3	74	51813	5.49	667	9	23	19	−4.8
1530	IPI00509014	α-Tropomyosin	272	32720	4.7	81	21	53	53	−4.8
1885	IPI00509014	α-Tropomyosin	79	32720	4.7	663	11	57	31	−6.3
1414	IPI00497758	Fast myosin heavy chain 4	71	222067	5.54	640	14	23	6	−6.6
1491	IPI00497758	Fast myosin heavy chain 4	92	222067	5.54	664	20	28	10	−7.6
1235	PI00483287	Fast skeletal myosin h. chain 3	117	51813	5.49	622	15	40	32	−8.3
2158	IPI00488085	Myosin light chain 2	216	18853	4.68	654	12	45	69	−8.6
1531	IPI00497758	Fast myosin heavy chain 4	92	222067	5.54	659	16	26	7	−9
1109	IPI00497758	Fast myosin heavy chain 4	100	222067	5.54	649	27	43	17	−11.1
1029	IPI00497758	Fast myosin heavy chain 4	131	222067	5.54	615	22	48	10	−11.2
1392	IPI00483287	Fast skeletal myosin h. chain 3	184	51813	5.49	613	7	61	23	−13.7
1133	IPI00483287	Fast skeletal myosin h. chain 3	103	51813	5.49	643	15	52	31	−14.2
1104	IPI00497758	Fast myosin heavy chain 4	79	222067	5.54	629	22	52	14	−15.4
1405	IPI00500057	Myosin, heavy polypeptide 2	66	221742	5.55	626	10	45	31	−24.5
2022	IPI00607465	Vg1 protein	276	36409	9.23	46	11	61	36	−44.4
2026	IPI00607465	Vg1 protein	179	36409	9.23	55	11	61	37	−82.4
2028	IPI00607465	Vg1 protein	264	36409	9.23	67	12	61	37	−40.5
2040	IPI00607465	Vg1 protein	225	36409	9.23	60	11	61	35	−7.3
2046	IPI00607465	Vg1 protein	161	36409	9.23	78	10	57	32	−6.7
1539	IPI00496717	Vitellogenin 1	68	149452	8.68	64	11	50	10	−10.6
1666	IPI00513217	Vitellogenin 1	88	21118	9	75	7	53	40	−16.1
1695	IPI00513217	Vitellogenin 1	65	21118	9	34	8	54	52	−5.2
1712	IPI00513217	Vitellogenin 1	130	21118	9	36	7	56	39	−14.9
1734	IPI00513217	Vitellogenin 1	84	21118	9	50	6	59	32	−192.6
									%
2022	IPI00496717	Vitellogenin 1	240	149452	8.68	46	14	56	11	−44.4
2026	IPI00496717	Vitellogenin 1	155	149452	8.68	55	16	56	14	−82.4
2028	IPI00496717	Vitellogenin 1	228	149452	8.68	67	16	55	12	−40.5
2040	IPI00496717	Vitellogenin 1	192	149452	8.68	60	15	56	12	−7.3
2046	IPI00496717	Vitellogenin 1	148	149452	8.68	78	16	51	15	−6.7
1624	IPI00507087	Muscle creatine kinase	75	42797	6.32	65	9	55	27	−5.7
1532	IPI00495855	l-Lactate dehydrogenase B	130	36089	6.43	48	11	39	36	−10.5
1423	IPI00490850	Aldolase c	193	39234	6.21	54	16	55	55	−34.1
1322	IPI00485952	Creatine kinase, mitochondr. 2	87	46265	6.49	19	9	33	24	−24.1
1231	IPI00490877	Eno3 protein	118	47402	6.2	20	13	57	45	−50.3
1363	IPI00508284	Ribosomal protein SA	74	33990	4.75	30	5	36	30	−12.9
1285	IPI00501593	RNA-binding protein 4	100	46105	6.81	18	10	16	29	−18
1285	IPI00615024	RNA binding motif protein 4	76	46089	6.81	17	8	16	23	−18
1501	IPI00496845	Eukar. transl. init. fact. 3	87	38572	5.53	73	11	44	40	−4
1495	IPI00491050	Het. nuclear ribonucleoprotein	78	37087	5.76	35	7	51	22	−3.7
1819	IPI00480889	Prohibitin	126	29666	5.28	37	9	60	38	−5
1029	IPI00498630	Chaperonin contain. TCP1 S5	125	59925	5.33	26	17	50	22	−11.2
1064	IPI00508003	Hspd1 protein	90	61157	5.56	32	12	30	29	−10.4
2062	IPI00495773	Crystallin, γN2	194	21730	5.86	660	11	47	62	−18.6
1921	IPI00502990	Crystallin, βB1	183	26797	6.44	52	10	58	46	−4
2002	IPI00490966	βA4-Crystallin	216	23013	6.25	40	13	58	85	−8.4
1979	IPI00504818	βA1–2-Crystallin	83	24521	6.4	77	9	62	67	−6.4
2478	IPI00513361	Novel β-type globin	111	11951	6.81	95	7	70	77	−3.2
2478	IPI00502256	Similar to embryonic 1	124	16151	6.89	95	8	68	63	−3.2
1531	IPI00498781	Similar to vertebrate APEX	97	34874	5.77	68	9	65	32	−9
2743	IPI00510181	Ubiquitin ribosomal prot. S27a	71	17987	9.68	89	5	37	30	9.3
2743	IPI00619743	Ubiquitin C	117	26486	7.85	89	5	37	40	9.3
2010	IPI00483436	NADH dehydrogenase	66	23687	5.74	104	8	56	38	5.3

^aGel spot number (also reference to supplemental Table 4).

^bIPI accession number.

^cMASCOT protein score.

^dMass spectrum ID (as reference to view the annotated mass spectrum in supplemental Table 4).

^eNumber of unique peptides matched to mass peaks.

^fNumber of unmatched mass peaks.

^gSequence coverage in percent.

^h-Fold change of normalized spot volume between embryonic stage (120 vs. 72 hpf).