Table 1.

Activation of p21^ras

Growth conditions*	Cell lines
	NIH 3T3	RhPV 1 E7	RhPV 1 E5	G54	pCO11
Steady state
Average (N) SEM†	1.18  (3) 0.09	1.33  (4) 0.31	6.88  (5) 0.59	9.47  (2) 0.16	7.20  (3) 1.13
Relative activation‡	1.0	1.1	5.8	8.1	6.1
Quiescent
Average (N) SEM	0.10  (2) 0.02	1.38  (4) 0.53	0.61  (4) 0.22	1.10  (2) 0.10	1.30  (3) 0.53
Relative activation‡	1.0	13.8	6.1	11	13
EGF-stimulated
Average (N) SEM	2.87  (4) 0.52	2.94  (4) 0.17	6.16  (5) 0.65	8.31  (2) 1.82	7.53  (3) 0.82
Relative activation‡	1.0	1.0	2.1	2.9	2.6
Stimulation effect§	28.7	2.1	10.1	7.6	5.8
PDGF-stimulated
Average (N) SEM	18.09  (5) 2.77	34.71  (3) 1.23	23.01  (3) 2.27	3.22  (2) 0.27	14.65  (3) 0.68
Relative activation‡	1.0	1.9	1.3	0.2	0.8
Stimulation effect§	180.9	25.2	37.7	2.9	11.3

^*Growth conditions tested were normal culture conditions (steady state), quiescent cells without EGF, and quiescent cells that had been stimulated with EGF or PDGF for 30 min. 

^†Activation of p21^ras was measured in independent assays. The average % GTP of these assays is presented with the number (N) of assays and the standard error of the mean (SEM). 

^‡Activation was compared to parental NIH 3T3 cells. 

^§Relative p21^ras activation of EGF- or PDGF-stimulated cells compared to their quiescent counterparts.