Figure 3.

Complex formation of mRGS-r protein with TDα_GDP·AMF analyzed by gel filtration. Purified TDα_GDP (6 μM) was incubated with 18 μM mRGS-r for 10 min at 30°C in the absence (○ and Middle) and presence of AMF (• and Bottom). Fractions in the protein profile (Top) were analyzed by SDS/PAGE, followed by Coomassie brilliant blue staining. Complex formation of mRGS-r protein with TDα was observed in the presence of AMF. The calculated molecular sizes were 40 kDa, 35 kDa, and 72.5 kDa for TD, mRGS-r protein, and the complex, respectively.