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. 1996 Nov 12;93(23):12896–12901. doi: 10.1073/pnas.93.23.12896

Figure 3.

Figure 3

Characterization of bRFC ATPase activity. (A) Stimulation of bRFC ATPase by DNA effectors. ATPase reactions were carried out as described. Reaction mixtures contained bRFC in amounts as indicated, in the absence or presence of 12.5 μM (nucleotide concentration) of the following DNAs: oligo(dT)12–18, poly(dA)4500, poly(dA)4500/oligo(dT)12–18 (20:1 nucleotide ratio), or φX174 circular ssDNA. (B) PCNA stimulation of RFC ATPase activity. PCNA was added in amounts as indicated to reaction mixtures containing 15 ng of bRFC in the presence of 12.5 μM of poly(dA)4500 or poly(dA)4500/oligo(dT)12–18. (C) HSSB stimulation of RFC ATPase activity. HSSB was added in amounts as indicated to reaction mixtures containing poly(dA)4500/oligo(dT)12–18 in the absence or presence of 10 ng of bRFC and/or 40 ng of PCNA.