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bRFC preferentially binds to DNA primer ends. Binding of bRFC to DNA was examined using a nitrocellulose filter binding assay as described. bRFC, in amounts as indicated, was incubated with poly(dA)300, oligo(dT)12–18, or poly(dA)300/oligo(dT)12–18 at a molar ratio of 1:0.4, 1:2 and 1:10, respectively, as indicated. The 100% value represented 40 fmol of input DNA.
