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. 1996 Nov 12;93(23):12926–12931. doi: 10.1073/pnas.93.23.12926

Figure 1.

Figure 1

Incorporation of [32P]AMP into polysomal RNA. Polysomes were isolated from SK5667 (PAP I+) and SK8964 (PAP I−) and incubated in the presence of [α-32P]ATP. Samples were removed at the following time intervals. Lanes 1, 4, 8, and 12, 0.3 min; lanes 5 and 13, 2 min; lanes 2, 6, 9, and 14, 5 min; lanes 3, 7, 10, and 15, 10 min; and lane 11, 15 min. After phenol extraction and ethanol precipitation, the RNA was resolved by 6% PAGE in the presence of 7 M urea. Exogenous PAP I was added at the rate of 1 unit/reaction to lanes 1–3. Polysomes isolated from SK8964(ΔpcnB) transformed with pJL89, a plasmid carrying the pcnB+ gene, were used in lanes 12–15. RNA size markers in nucleotides are indicated on the right.