Figure 1.

Overexpression of PTPMEG in COS-7 cells. (A) Immunoblot of PTPMEG in COS-7 cell clones. Sixty-four micrograms of 1% Triton extract from each clone was separated by SDS/8% PAGE, transferred to nitrocellulose, and blotted with anti- PTPMEG antiserum. (B) PTPMEG tyrosine phosphatase activity in overexpressing COS-7 cell clones. PTPMEG activity in 30 μg of the lysate was analyzed with the immunocomplex assay as described in experimental procedures. V, clones transfected by pCEN vector alone; N, clones transfected with PTPMEG; M, clones transfected with inactive PTPMEGCS.