Table 2.
The growth parameters of the PTPMEG and PTPMEGCS stable clones
| Clone no. | PDT, hr | Cell density in plateau stage, ×105 per cm2 | Saturation cell density, ×105 per cm2 | Colonies in soft agar
|
|
|---|---|---|---|---|---|
| Number* | Size | ||||
| V1 | 21.6 (r = 0.98) | 6.84 ± 0.379 | 8.07 ± 0.619 | 6,774 ± 162 | Medium |
| V2 | ND | ND | 8.72 ± 1.092 | 13,836 ± 756 | Large and medium |
| V6 | 25.7 (r = 0.98) | 4.74 ± 0.063 | 6.41 ± 0.411 | 4,974 ± 270 | Medium |
| N25 | 31.6 (r = 0.99) | 2.42 ± 0.045 | 2.78 ± 0.160 | 360 ± 60 | Small |
| N30 | 27.5 (r = 0.98) | 2.63 ± 0.028 | 2.88 ± 0.393 | 198 ± 42 | Small |
| N37 | 27.4 (r = 0.98) | 3.22 ± 0.089 | 4.06 ± 0.344 | 204 ± 36 | Small |
| M37 | 24.0 (r = 0.97) | 5.45 ± 0.148 | 5.82 ± 0.488 | 384 ± 48 | Small |
| M71 | 24.0 (r = 0.97) | 5.29 ± 0.221 | 4.85 ± 0.154 | 390 ± 30 | Small |
| M73 | 23.7 (r = 0.99) | 5.14 ± 0.108 | 5.24 ± 0.466 | 1,824 ± 72 | Medium |
Colonies in soft agar were counted directly using an inverted microscope. The population doubling time (PDT) was measured by least mean square analysis of the cells during log phase growth. The cell density at plateau is determined using the cell number on the last day of the growth curve. The saturation density is the mean of the last three values. All points are the mean of triplicates ± SD. ND, not determined.
*
Colony number per 60-mm dish.