Figure 1.
Karyopherin β is bound by α1–110 and α1-61, but not by α107-542 and α62-542. (A) Schematic drawing of karyopherin α and fragments that were isolated as recombinant GST fusion proteins from E. coli. The N-terminal (N), the armadillo repeat (A), and the C-terminal (C) regions are indicated. (B) Immobilized GST-α1–110 (lane 1), GST-α107-542 (lane 2), GST-α1-61 (lane 3), and GST-α62-542 (lane 4) were incubated with recombinant karyopherin β. Bound fractions were analyzed by SDS/PAGE and Coomassie blue staining. The immobilized GST-tagged fragments are marked by asterisks.