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In vivo association of karyopherin α with in vivo-expressed β288-457. Following galactose induction for 4 h, GST-β288-457-ProtA was isolated from yeast cytosol by IgG Sepharose chromatography. The bound fraction was analyzed by SDS/PAGE and Coomassie blue staining (lane 1) or ECL Western blotting using anti-α antibodies and protein A-horseradish peroxidase conjugates (lane 2) or anti-rabbit IgG-HRP conjugates (lane 3).
