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Ran-GTP is bound by β1-861, β1-357, β1-457, and β288-457, but not by β1-288 and β348-861. Equal amounts of the fusion proteins GST-β1-861 (lane 1), GST-β1-288 (lane 2), GST-β1-353 (lane 3), GST-β1-457 (lane 4), GST-β348-861 (lane 5), and inclusion bodies containing GST-β288-457 (lane 6) were separated by SDS-PAGE and subjected to a Ran-[α-32P]GTP ligand blot according to Coutavas et al. (32). Labeled proteins were visualized by autoradiography.
