Figure 8.
Expression of α1-61 and β288-457 in yeast inhibit nuclear import of a coexpressed nuclear reporter protein. Cells were grown overnight in dextrose, shifted to 2% galactose for 10 h, and then directly examined by fluorescence microscopy (A) or Nomarski optics (B). Shown are cells expressing the galactose inducible fusion protein consisting of the naturally GFP and histone H2B (28) and protein A (1), α1-61-ProtA (2), or GST-β288-457-ProtA (3). All micrographs were exposed for the same length of time.