Table 1.

Effects of T channel antagonists on the zona pellucida-induced acrosome reaction

Treatment	Conc.	Acrosome reacted, %
		+Control	+ZP
Medium	—	23  ±  8	62 ± 6*
Pimozide	0.1 μM	21  ±  3	49 ± 5*
	1.0 μM	18  ±  6	27 ± 4†
Amiloride	0.1 mM	17  ±  4	54 ± 7*
	0.5 mM	22  ±  3	33 ± 4*†

Capacitated mouse sperm were incubated for 10 min in culture medium or in medium supplemented with the indicated T channel antagonists. The fraction of acrosome reacted sperm in culture medium-treated populations was 19%  ±  5%, and this background value was not altered significantly by treatment with T channel antagonists (range of means for pimozide and amiloride, 16–20%). Sperm were incubated an additional 20 min after addition of control glycoproteins (+Control, 50 μg/ml transferrin or fetuin) or soluble extracts of zonae pellucidae (+ZP, 50 μg/ml) were added and acrosome reactions were assessed. The results are given as the mean (±SEM) of triplicate experiments, with triplicate samples obtained in each experiment and with 100–200 sperm assayed per sample. 

^*Sperm treated with zonae pellucidae differ from sperm treated with control glycoproteins (P < 0.01). 

^†Sperm treated with T channel antagonists differ from culture medium controls (P < 0.01).