Table 1.
In vitro development of identical mouse eggs reconstructed with four-cell nuclei synchronized at metaphase
| Identical sets | No. of eggs
examined
|
No. of reconstructed eggs developed to
|
|||||
|---|---|---|---|---|---|---|---|
| 1st NT* | 2nd NT† | Two-cell | Four-cell | Eight-cell | Morula | Blastocyst | |
| i | 4 | 8 | 8 | 7 | 7 | 7 | 7 |
| ii | 4 | 8 | 8 | 7 | 7 | 7 | 7 |
| iii | 4 | 8 | 8 | 6 | 5 | 5 | 5 |
| iv | 4 | 7 | 7 | 7 | 6 | 6 | 3 |
| v | 4 | 7 | 7 | 7 | 7 | 7 | 7 |
| vi | 4 | 7 | 7 | 6 | 6 | 6 | 6 |
| vii | 4 | 6 | 6 | 6 | 5 | 5 | 5 |
| viii | 4 | 6 | 6 | 6 | 6 | 6 | 6 |
| ix | 4 | 5 | 5 | 5 | 5 | 5 | 5 |
| x | 4 | 4 | 4 | 3 | 3 | 3 | 3 |
| xi | 3 | 4 | 4 | 4 | 4 | 4 | 4 |
| Total | 43 | 70 | 70 | 64 | 61 | 61 | 58 |
| (%) | (100) | (91) | (87) | (87) | (83) | ||
NT, Nuclear transfer.
*
Nuclear transferred eggs formed two pronuclei.
†
After the first NT, each nuclei was transferred into enucleated fertilized zygotes obtained from superovulated mated females.