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. 2007 Dec 10;26(6–7):815–820. doi: 10.1080/15257770701503787

FIGURE 1.

FIGURE 1

Intracellular localization of triple-transfected expression vectors in Huh-7 cells. Plate-A: Triple transfection of pU6-shRNA-452, pGFP-core (nuclear exporting vector), and pDsRed-PA28γ vectors into Huh-7 cells. Cleavage of the GFP-core protein mRNA, which is the target of shRNA-452, generated expression only in the cytosol in the GFP image, while both the DsRed and merged images show nuclear expression. Plate-B: Triple transfection of the pU6-shRNA-479, pGFP-core, and pDsRed-PA28γ vectors into Huh-7 cells. Cleavage of GFP-core protein mRNA, which is the target of shRNA-479, generated expression only in the cytosol in the GFP image, while both the DsRed and the merged images show nuclear expression. Plate-C: In a similar fashion, pU6-shRNA-503, together with pDsRed-PA28γ and pGFP-core mRNA, expressed exhibited cytoplasmic localization in the GFP image. Plate-D: The scrambled version of all of the pU6-shRNAs was transfected with the pDsRed-PA28γ and pGFP-core vectors. Nuclear expression is shown in the GFP, DsRed, and merged images.