Figure 3.

Hydrophatic profiles of cyprinid hedgehog exon 2 paralogues. PCR primers designed to amplify Shh exon 2 within cyprinids, also amplified two other exon 2 paralogues [Ihh and Dhh according to the nomenclature of Echelard et al. (2)]. The hydrophilicity of the deduced amino acid sequences for all exon 2 paralogues was calculated by the Kyte–Doolittle method (36) using the macvector program (IBI). Secondary structures (19) assigned to Shh exon 2 are shown. Coordinating and noncoordinating residues likely to be important for catalysis are indicated by an asterisk. Positions that characterize each of the paralogues are indicated by a dot.