Table 2.
Strain | Alkaline phosphatase activity* | ompA† | Motility | Cu2+ resistance‡ | DsbC dependence§ | Locus | No. of mutants |
---|---|---|---|---|---|---|---|
Wild type | 159.3 ± 12.4 | + | ++++ | + | |||
dsbA::kan | 4 ± 1.8 | + | — | + | |||
Class I | 7.9 ± 1.6 | + | + | — | Yes | dipZ− | 5 |
Class II | 22.2 ± 4.9 | — | +++ | — | Yes | dipZ− | 2 |
Class III | 10.5 ± 4.9 | + | + | −/+ | Yes | trxA− | 1 |
Class II/dsbC− | 2.2 ± 1.6 | — | |||||
Class III/dsbC− | Not assayed | — |
Phosphatase assays were performed as described in Materials and Methods on strains carrying the phoA68 signal sequence mutation.
Indicates whether or not the strains contain an intact ompA gene.
Cu2+ resistance refers to the ability of the strain to grow in the presence of 4 mM cupric sulfate.
DsbC dependence indicates whether suppression depends on the presence of a functional dsbC allele.