Table 1.
Correlation between gene conversion levels and the amount of DSBs
| Gene conversion
|
DSB
measurements
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Diploid | Genotype | No. of tetrads | GC content, % | Diploid | Genotype | No. of experiments | DSB, % | ||
| RM169 |
|
391 | 14.1 | RM207 |
|
5 | 4.3 ± 0.8 | ||
| RM198 |
|
319 | 23.8 | RM206 |
|
3 | 12.2 ± 0.6 | ||
| RM187 |
|
316 | 2.2 | RM216 |
|
2 | <0.5* | ||
The percent DSB = (the counts in the DSB bands divided by the total number of counts) × 100. RM207(rad50S/rad50S) is congenic to RM169; RM206 (rad50S/rad50S) is congenic to RM198; and RM216 (rad50S/rad50S) is congenic to RM187. DSBs were measured at 10 and 12 hr. The DSB values presented are the average of the number of experiments shown.
*
No bands at the normal DSB positions were detected in two independent experiments. Reconstruction experiments indicate that bands consisting of 0.5% of the total DNA could be detected.