Table 3.

DSBs in diploids heterozygous for the Aha deletion

Experiment (strain)	Chromosome broken		10 hr		12 hr		Average ratio hot/cold ± SD
			DSB, %	Ratio, hot/cold	DSB, %	Ratio, hot/cold
1 (RM204)	Cold	B	2.67		2.61
		C	1.99		2.02
		(B+C)	4.66		4.63
				1.09		1.03	1.06 ± 0.04
	Hot	B′	2.95		2.73
		C′	2.11		2.05
		(B′+C′)	5.06		4.78
2 (RM204)	Cold	B	2.76		2.63
		C	2.20		2.18
		(B+C)	4.96		4.81
				1.00		1.06	1.03 ± 0.04
	Hot	B′	2.72		2.76
		C′	2.25		2.35
		(B′+C′)	4.97		5.11
3 (RM204)	Cold	B	2.28		2.02
		C	1.70		1.60
		(B+C)	3.98		3.62
				0.92		1.04	0.98 ± 0.08
	Hot	B′	2.33		2.62
		C′	1.34		1.16
		(B′+C′)	3.67		3.78
4 (RM214)	Cold	B	2.27		3.25
		C	1.99		2.43
		(B+C)	4.26		5.68
				0.97		0.91	0.94 ± 0.04
	Hot	B′	2.30		3.07
		C′	1.81		2.12
		(B′+C′)	4.11		5.19
Overall average				1.00 ± 0.07		1.01 ± 0.07	1.00 ± 0.2

RM204 has the genotype his2-xho-ΔAha/his2-NSI rad50S/rad50S and is congenic to RM169. RM214 has the genotype his2-xho-ΔAha/HIS2 rad50S/rad50S and is isogenic to RM169. The hot chromosome refers to the homolog containing the Aha deletion; the cold chromosome refers to the homolog without the Aha deletion. B, B′, C, and C′ are defined as in Fig. 5. The times refer to cells taken at 10 hr and 12 hr after introduction into sporulation medium. The ratio hot/cold represents the total percent of DSBs on the hot chromosome divided by the total percent on the cold chromosome. A hot/cold ratio of 1.0 indicates that DSBs were formed equally on both homologs.