Figure 2.

(A) Blot analysis of tail DNA prepared from wild type (+/+), Atm heterozygous (+/−), and homozygous (−/−) mutant mice. DNA was cut with EcoRI and probed with probe A (Fig. 1B). (B) Blot analysis of poly(A)⁺ selected RNA from thymi of wild-type (WT) and Atm-deficient (KO) mice. The blot was probed with a mouse Atm cDNA fragment as described. Each lane contained 2 μg of RNA. Equal loading was verified by ethidium bromide staining (not shown). (C) Blot analysis of proteins extracted from fibroblasts derived from healthy (C) or AT (ATM) humans and from thymi (Thy.) and spleens (Spln.) of wild-type and Atm-mutant mice.