Table 3.
Reduced T cell population in thymus, spleen, and peripheral blood from mutant Atm homozygous mice
| Tissue | Genotype | Thy1 | CD3 | αβTCR | CD4−CD8− | CD4+CD8− | CD4−CD8+ | CD4+CD8+ |
|---|---|---|---|---|---|---|---|---|
| Thymus | Control | 98.8 ± 0.6 | 20.3 ± 1.5 | 18.0 ± 1.7 | 2.8 ± 1.6 | 12.1 ± 0.5 | 8.9 ± 1.6 | 78.0 ± 2.3 |
| Atm | 98.5 ± 0.4 | 5.0 ± 1.3* | 5.4 ± 1.3* | 2.9 ± 0.9 | 3.6 ± 0.9* | 4.6 ± 1.4 | 88.9 ± 3.0** | |
| Spleen | Control | 33.7 ± 1.7 | 41.6 ± 1.4 | 44.7 ± 1.7 | 60.2 ± 1.9 | 25.0 ± 1.6 | 14.5 ± 0.7 | 0.2 ± 0.1 |
| Atm | 10.0 ± 1.5* | 15.6 ± 1.9* | 20.0 ± 1.8* | 85.5 ± 1.8* | 8.8 ± 1.2* | 5.4 ± 1.4* | 0.2 ± 0.1 | |
| Blood | Control | 39.7 ± 4.6 | 75.4 ± 3.1 | ND | 28.3 ± 2.1 | 54.9 ± 1.7 | 16.6 ± 0.8 | 0.2 ± 0.1 |
| Atm | 15.9* | 34.6* | ND | 45.1** | 37.3* | 17.6 | 0.1 |
Shown are the percentage of cells staining positive for each surface molecule by flow cytometry in Atm homozygotes and control (Atm heterozygote and wild-type) mice. n = 3 in all samples except in ATM blood, where n = 2. ND, not determined. ∗, P < 0.01; ∗∗ 0.01 < P < 0.05 by Student’s t test analysis.