Figure 3.

Expression of granzymes in LAK cells derived from wild-type (WT), granzyme B −/−, and cathepsin G −/− mice. LAK cell total RNA (20 μg), generated from WT, B −/−, and cathepsin G −/− mice was analyzed for the expression of granzymes A–G using S1 nuclease protection assays. Equivalent levels of granzyme A mRNA in all three samples indicates that all samples were similarly activated. Note that levels of granzymes C, F, and D mRNAs are substantially reduced in LAK cells derived from granzyme B −/− mice. These experiments were performed three times, yielding identical results. Autoradiograms for granzyme A, B, C, F, and D panels were exposed overnight. Granzyme G and E panels were exposed for 72 h.