Table 1.
Specificity and dose-dependency of the LTβR–Fc chimeric fusion protein
| Mouse* | Tg† | LTβR–Fc‡, μg/ml | Relative inhibition of cytokine
cytotoxicity§
|
Body, g | Spleen, g | Thymus, g | Peyer’s patches
|
|||
|---|---|---|---|---|---|---|---|---|---|---|
| LTαβ | LTα | TNF | n | cm2 | ||||||
| 1610.20¶ | + | 0.05 | 1.1 | <1 | <1 | 19.5 | 0.093 | 0.128 | 11 | 0.35 |
| 1610.30 | + | 0.10 | 1.6 | <1 | <1 | 20.3 | 0.084 | 0.097 | 8 | 0.31 |
| 1610.1 | + | 0.50 | 6.7 | <1 | <1 | 19.8 | 0.078 | 0.121 | 10 | 0.15 |
| 1610.3 | + | 0.50 | 5.8 | <1 | <1 | 17.1 | 0.055 | 0.089 | 7 | 0.16 |
| 1610.10 | + | 1.25 | 18.3 | <1 | <1 | 17.8 | 0.055 | 0.084 | 5 | 0.09 |
| 1610.2 | + | 1.30 | 19.2 | <1 | <1 | 14.5 | 0.049 | 0.113 | 5 | 0.07 |
*
Mice were 6-week-old female littermates born from heterozygous parents.
†
Expression of the LTβR–Fc transgene was determined by Southern blot analysis.
‡
Concentration of the soluble LTβR–Fc fusion protein in the sera was determined by ELISA.
§
Determined by the ability of 1% sera to inhibit either LTαβ-, LTα-, or TNF-mediated killing of WEHI-164 cells; 10 ng/ml of purified murine LTβR–Fc fusion protein in 1% NMS was able to inhibit LTαβ-killing by 19.2-fold.
¶
Similar values were observed with age- and sex-matched nontransgenic mice.