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Western blot of immunoprecipitated Aβ species. Conditioned media collected from the cell lines that express wt PS1, PS1-D385TG or PS1-M146V and were treated with either DMSO or 100 μM Sulindac Sulfide. Equal amount of the media was immunoprecipitated with monoclonal antibody 4G8 and the extensively washed immunoprecipitates were resolved on a Tricine-Urea gel. The blot was reacted with antibody 6E10 for detection.
