Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 May 12;3:6. doi: 10.1186/1750-1326-3-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2008 Carter et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

PS1 carrying D385TG triplet suppresses NICD production. Each indicated stable cell was transfected with the Notch ΔE-GVP, pFR-Luc and pRL-CMV. In Notch ΔE-GVP, the Notch ΔE protein was fused to VP16 transactivating protein domains (GVP). After the cleavage of the N ΔEGVP by the γ-secretase, the Notch ΔE-GVP intracellular domain will translocate to the nucleus and binds to the GAL4 DNA binding domain located in the upstream of the reporter pFR-Luc to activates transcription. The results are the average of the three experiments.