Table 2.

Levels of Ras and JAK/STAT Inhibitors in GNMT-Knockout Mice

Gene	Magnitude of change in GNMT-KO versus WT mice at 3 months	P value	Magnitude of change in GNMT-KO versus WT mice at 8 months	P value
SOCS 1	−2.1	0.007	−1.6	0.040
SOCS 2	−1.8	0.027	−2.5	0.002
SOCS 3	−2.2	0.021	−4.3	0.001
CIS	−2.2	0.017	−4.1	0.040
RASSF 1	−1.9	0.042	−1.6	0.024
RASSF 4	−1.7	0.0003	−2.4	0.010

Levels of JAK/STAT (SOCS1, SOCS2, SOCS3, CIS) and Ras (RASSF1 and RASSF4) inhibitors were determined by quantitative RT-PCR in the livers of wild-type (WT) mice at 3 and 8 months of age, in liver samples from 3-month-old GNMT-knockout (GNMT-KO) mice, and in liver tumors from 8-month-old knockout mice, normalized using GAPDH expression as a housekeeping gene, and the ratio (magnitude of change) of GNMT-KO versus WT calculated. Data represent means of at least 5 animals per subgroup assayed in triplicate.