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PCR primer pairs used for DNA sequencing and size of the fragments amplified
| Primer pair | Positiona | Length |
|---|---|---|
| HE3026/HE3027 | −2312 to −1577 | 736 (no indel); 1566 (with indel) |
| HE3028/HE3029 | −1647 to −928 | 720 |
| HE3040/HE3041 | −1118 to −381 | 738 |
| HE3032/HE3033 | −428 to 453 | 881 |
aPosition relative to the translational start site of OPRK1 (NM_000912) on the NCBI reference genome (NT_008183.18).
