Table 3.

OPRK1 constructs for transfection assays

Name	Positiona	Primer pairs	DNAb	Length (bp)
OPRK-1.6	−1647 to −10	HE3059/HE3061	5003	1638
OPRK-2.3	−2312 to –10	HE3058/HE3061	5003	2303
OPRK-3.1	−2312 to –10c	HE3058/HE3061	6006c	3133c

Fragments noted were cloned into pXP2 for assays of promoter activity.

^aPosition relative to the translational start site of OPRK1 (NM_000912) on the NCBI reference genome (NT_008183.18).

^bIndividual genomic DNA used as template.

^cIndividual 6006 has DNA with the indel; amplification using the same primers produced a fragment with the additional 830 bp indel.