Fig. 3.

Cultured AIDS-KS cells express both NOS 3 and NOS 2 isoforms. Representative RT–PCR analyses from two AIDS-KS strains confirm the presence of NOS 3 message during all culture conditions. While NOS 2 message was not present during log growth, NOS 2 was expressed following either sera deprivation or TNF-α challenge. (Lanes 1 and 4 = log growth, 2 and 5 = sera deprivation, 3 and 6 = 6 h challenge with 100 U/ml TNF-α.)