Fig. 3.
BMS-214662 inhibits cPLA2γ-mediated arachidonic acid release from IMLF−/−. IMLF−/− were infected with Ad-cPLA2γ and Ad-green fluorescent protein (GFP) control virus (upper panel) or Ad-GFPcPLA2α and Ad-GFP control virus (lower panel) and incubated with or without 1 μM BMS-214662 in medium containing [3H]arachidonic acid. The cells were washed and then stimulated with 10% mouse serum for 3 h. The amount of radioactivity released into the medium is expressed as a percentage of the total incorporated radioactivity. Data shown are the average ±SEM of three independent experiments.