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. Author manuscript; available in PMC: 2008 May 30.
Published in final edited form as: J Lipid Res. 2005 Aug 1;46(10):2122–2133. doi: 10.1194/jlr.M500230-JLR200

Fig. 9.

Fig. 9

Effect of N-terminal tags on A23187-stimulated [3H]arachidonic acid release from Spodoptera frugiperda (Sf9) cells expressing cPLA2γ. [3H]arachidonic acid-labeled Sf9 cells expressing cPLA2γ (circles), 6× histidine-tagged cPLA2γ (diamonds), GFP-cPLA2γ (triangles), or empty vector (squares) were treated with either vehicle (DMSO; open symbols) or 2 μg/ml A23187 (closed symbols) for the times shown. The amount of [3H]arachidonic acid released into the medium is expressed as a percentage of the total incorporated radioactivity. Data shown are averages ± SD of triplicate samples of one experiment that is representative of three independent experiments.