Figure 2. Recombination induced at the early pupal stage: fluorescence analysis in the Drosophila wing after adult emergence.

Analysis of fluorescence in the anterior wing margin after emergence of the adult fly (1–6): Progenies of the A+B cross (see Materials and Methods) were heat-shocked at the early pupal stage and the adult wings were analyzed for GFP fluorescence. (1–4) Representative images of two typical wings are shown. Note the alignment of the fluorescent cells, and that there are no axons visible as yet (alignment of “boutons”). Note also the disparity between differentiation processes in the same wing. (5) Heavily fluorescent dorsal row of sensilla along the wing margin. (6) Another typical wing shows two dorsal rows of fluorescence: one is intense, the other weak. The latter weak row does not exist in (5), although the wings are of roughly the same age. (see photo 1 in this panel and figure 1 photo 6 for dimensions and orientation). Analysis of fluorescence in the median vein III of the adult wing at between 5 and 10 hours after emergence (7–10): (7–10) Campaniform sensilla along vein III of an adult wing. One sensilla has four labeled cells, another shows two labeled cells, and two other sensilla show one labeled cell undergoing division (planar anaphase). (see photo 1 in this panel and figure 1 photo 6 for dimensions and orientation).