Table 2. Purkinje Cell Dendritic Defects in Bcl-w^−/−Grid2^{ho−4J(−/−)} Mice.

Genotypes	Number of Purkinje Cell Branches in Each Strahler Order
Wild Type	I	II	III	IV	V	VI
Cell 1	319	84	21	5	2	1
Cell 2	201	56	15	5	2	1
Cell 3	289	81	21	5	2	1
Cell 4	221	65	20	7	2	1
Mean±S.E.M	257.5±27.8	71.5±6.3	19.3±1.4	5.5±0.5	2	1
Bcl-w−/−
Cell 1	199	56	21	6	2	1
Cell 2	192	59	17	5	2	1
Cell 3	230	66	20	6	2	1
Cell 4	238	64	21	7	2	1
Mean±S.E.M	†214.8±11.3	*61.3±2.3	19.8±0.9	‡6.0±0.4	2	1
Grid2ho−4J(−/−)
Cell 1	321	85	24	5	2	1
Cell 2	313	94	26	5	2	0
Cell 3	250	66	17	4	2	1
Cell 4	271	67	21	5	2	0
Mean±S.E.M	†288.8±16.9	*78±6.9	22±2.0	‡4.8±0.3	2	0.5
Bcl-w−/−Grid2ho−4J(−/−)
Cell 1	60	13	3	1	0		0
Cell 2	97	25	6	2	1		0
Cell 3	88	26	6	1	0		0
Cell 4	99	27	6	3	1		0
Cell 5	64	22	5	1	0		0
Cell 6	107	27	7	2	1		0
Mean±S.E.M	85.8±7.9	23.3±2.2	5.5±0.6	1.7±0.3	0.5±0.2		0

Purkinje cell dendritic abnormalities in Bcl-w^−/−Grid2 ^{ho−4J(−/−)} mice were quantified using the Strahler system of topological analysis on Golgi-stained Purkinje cells. The number of the dendritic branches in each Strahler order was counted for several Purkinje cells for wild type, single knockout, and double knockout mice. Statistic analysis was performed between two genotypes of mice in each order using student t-test. Purkinje cells in Bcl-w^−/−Grid2^{ho−4J(−/−)} mice are significantly different from all other genotypes in every Strahler order (p<0.01). ^†, ^*, ^‡ Indicate the significant difference (p<0.05) in the first, the second, and the fourth Strahler order between Bcl-w^−/− and Grid2^{ho−4J(−/−)} mice.