Table 1.
Breakthrough of mutant HIV-1/IIIB strains in CEM cell cultures in the presence of 3TC
| Mutant RT virus strain | 3TC concentration, μg/ml | Mean day of virus breakthrough (50% cytopathicity) | Additional amino acid mutation in the RT |
|---|---|---|---|
| 103-Asn | 0.5 | 17 | ND |
| 2.5 | 28 | 103-Asn + 184-Ile/Thr | |
| 106-Ala | 0.5 | 26 | ND |
| 138-Lys | 0.5 | 13 | 138-Lys + 184-Ile |
| 2.5 | 19 | 138-Lys + 184-Ile | |
| 181-Cys* | 0.5 | 36 | 181-Cys + 184-Ile |
| 2.5 | >45† | NA | |
| 181-Cys* | 0.5 | 22 | 181-Cys + 184-Ile |
| 2.5 | >45 | NA | |
| 190-Glu | 0.05 | >45 | NA |
| 0.2 | >45 | NA | |
| 0.5 | >45 | NA | |
| 2.5 | >45 | NA | |
| Wild type | 0.5 | 22 | ND |
| 2.5 | 32 | 184-Val | |
| 0 | 2.5 | NA |
ND, not determined; NA, not applicable.
*
These 181-Cys mutant viruses represent two independent isolates.
†
At day 45 of the experiment (13th passage), no cytopathicity was detectable. Therefore, the value of mean day of virus breakthrough was indicated as >45.