Figure 5.

Gene expression of transcription factors in individual quiescent multipotent hematopoietic cells (G₀) or CD34⁺,CD38⁻ cells analyzed using the single cell RT-PCR technique. The ethidium bromide (EtBr)-stained cellular cDNA gel was subsequently transferred to a nylon filter, probed with the indicated cDNA probes, and subjected to autoradiography. Panels of five cells of each type were used to avoid individual cell artifacts.