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. 1996 Nov 12;93(23):13164–13169. doi: 10.1073/pnas.93.23.13164

Figure 1.

Figure 1

Cell growth and survival rate in the alveolar RMS cell line Rh30 upon treatment with specific PAX3 antisense oligonucleotides. Rh30 cells express the aRMS specific translocation product PAX3/FKHR. ODN are directed against the translational start site of the PAX3 mRNA. (a) Growth rate of Rh30 cells incubated with different concentrations of antisense PAX3 (as-P3, solid symbols) and sense PAX3 (s-P3, open symbols) ODN. Concentrations used were 0.01 μM (▪), 0.1 μM (•), and 1 μM (▴). Viable cells were counted every 24 hr for five consecutive days. (b–d) Viability of Rh30 and control cells incubated with increasing concentrations of antisense (solid bars), missense (shaded bars), or sense (open bars) ODN. The number of cells treated with lipofectin only is indicated by the horizontal lines (dashed lines). Rh30 cells were treated with PAX3 ODN (b) and PAX7 ODN (d), control MRC-5 human lung fibroblasts were treated with PAX3 ODN (c). Cells were counted after 5 days except for in d where they were counted after 3 days. Results shown represent means of at least three independent experiments each using three dishes for every time point counted. Bars = SD.