Figure 1.

MDL 28170 influences βAPP metabolism. 0, Untreated K695sw cells. 200, K695sw cells treated with 200 μM MDL 28170. Lanes: 1 and 2, aliquot of total chase medium electrophoresed directly on the gel; 3 and 4, 1736 immunoprecipitation of chase medium; 5 and 6, 192sw immunoprecipitation of chase medium [note that β-cut APPs (lanes 5 and 6) runs slightly below α-cut APPs (lanes 3 and 4), as expected]; 7 and 8, C7 immunoprecipitation of cell lysates (N′ and N′/O′-glycosylated forms of full-length βAPP as well as the 10-kDa C-terminal fragment are indicated); 9 and 10, 1282 immunoprecipitation of cell lysates.