Figure 3.

Differential inhibition of Aβ₄₂ and Aβ₄₀ formation in K695sw cells under a variety of conditions. (A) Treatment with 1 μM phorbol 12,13-dibutyrate (lanes P) decreases both Aβ₄₂ and Aβ_total. (Upper) 21F12 precipitation. (Lower) Subsequent 1282 precipitation. (B–E) Cells were chased with (lanes M) or without (lanes 0) 200 μM MDL 28170 and the resultant media were immunoprecipitated as follows. (B) First with BC05 (Upper) and then with 1282 (Lower). (C) First with C42 (Upper) and then with 1282 (Lower). (D) First with 2G3 (Upper) and then with 21F12 (Lower). (E) In two separate aliquots, either with 21F12 (Upper) or with 1282 (Lower). (F) Cells were labeled for 3 h in the presence of 100 μM MDL 28170 and the medium was immunoprecipitated first with 21F12 (Upper) and then with 1282 (Lower). (G) Cells were chased with (lane M) or without (lane 0) 200 μM MDL 28170 and the resultant media were immunoprecipitated first with 21F12 (Upper) and then with 1963 (Lower).