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. 2003 Oct 14;100(22):12543–12547. doi: 10.1073/pnas.2035257100

Fig. 2.

Fig. 2.

CV of 800 μM MutY at a gold surface modified with the thiol-terminated duplex SH-5′-AGTACAGTCATCGCG and passivated with mercaptohexanol. (a) The redox potential of MutY measured in these experiments requires DNA binding. The CV of MutY at a surface modified with mercaptohexanol (MCH) only (black) shows no redox process, whereas at a DNA/MCH-modified Au surface (blue), the DNA-bound redox potential of MutY is ≈75 mV vs. Ag (≈275 mV vs. NHE). (b and c) This electrochemical signal grows in with DNA-binding time (CVs for incubation from 1–10 min) (b) and persists as the MutY solution is exchanged for pure buffer (20 mM sodium phosphate, pH 7.5/1 mM Na2EDTA/20% glycerol/500 mM NaCl) (c), indicating that MutY is bound to the DNA-modified surface.