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Inhibition by auranofin. Auranofin, an effective inhibitor of human TrxRs, was analyzed for its inhibitory effect on wild-type DmTrxR (highlighted in black) and the DmTrxR mutants. Shown is the percentage of inhibition compared with an auranofin-free control. In all cases, 1 μM auranofin was used and the assays were carried out as in Fig. 1.
