Figure 3.

Northern blot analysis for (A) NADPH oxidase subunit gp91^phox and (B) KH₂O₂ channel subunit (KV3.3a). Lanes: 1, human mononuclear cell RNA extract (control); 2, human lung; 3, fetal rabbit lung; 4–9, SCLC cell lines (H-727, H-345, H-128, H69V, H-69, and H-146). Each lane has 15 μg total RNA and was analyzed by Northern blotting and hybridized with ³²P-labeled gp91^phox and KV3.3a RNA antisense probe, respectively. In both cases the expected mRNA size of ≈4.7 kb for gp91^phox and ≈4.8 kb for KV3.3a was detected as described in previous reports (9, 12).