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Increase of capped HIV-1 transcripts coupled with accumulation of Ser-5 phosphorylation in infected cells. OM10.1 cells were treated with TNFα to induce virus production and then subjected to ChIP assays. Transcription complexes were immunoprecipitated with antibody α-CTD H5 or H14 or α-2,2,7-trimethylguanosine, and recovered DNA was used for PCR amplification of the HIV-1 and GAPDH promoter regions.
