Table 1. Characteristics of the cascading networks used in this study.

No. of stages	Cascade	Time delay, min	Maximum Luc production, nM
1	T7-luc	15	500
	SP6-luc		2,000
2	T7-SP6rnap → SP6-luc	60-90	100
	SP6-T7rnap → T7-luc		100
3	T7-SP6rnap → SP6-σ28 → Ptar-luc	180	1