Figure 4.

Short contact with PCs leads to proliferative inhibition of RCs. Primary macrophages were separated by adhesion from PBMCs, infected with MV (MOI 3 for 2 days until they were 80% positive for MV antigens) (MV-M) and UV-irradiated. For each value, 1 × 10⁶ MV-M were mixed with 2 × 10⁶ RCs in the presence of PHA and the PCs were separated by anti-CD14 magnetic cell sorting selection after 10 min (lane 1), 30 min (lane 2), or 60 min (lane 3) from the RCs. RCs were further cultivated in the absence of PCs for 72 hr and subsequently labeled for 16 hr.