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Gene-specific RT-PCRs on RNA prepared from dissected M. confertiflora flower buds. Adaxial, lateral, and abaxial corolla and connate (attached) stamens were dissected from later stage buds (Fig. 1e). Sepal tissue was used as a negative control of CYC and DICH expression. Actin primers were used as a positive control. Locus specificity was confirmed by sequence analysis of RT-PCR products.
