Fig. 2.

Detection of β3Gal-T5 transcripts initiating within the LTR and native promoters. (A) Total cDNAs derived from a range of normal human tissues were used in RT-PCR assays, with primers specific for GAPDH, the β3Gal-T5 ORF, and β3Gal-T5 transcripts initiating within the LTR or native promoter. Approximate molecular weights are indicated on the left. (B) Southern blot of the gel shown in the third panel in A. The blot was probed with a labeled oligonucleotide specific for β3Gal-T5 exon 4.