Fig. 4.

Contribution of the LTR promoter to β3Gal-T5 expression in human tissues. Primers were used in real-time PCR assays to amplify transcripts specific for GAPDH, total β3Gal-T5, and β3Gal-T5 transcripts initiating within the LTR promoter from cDNAs derived from normal human tissues. Gray bars represent the relative abundance of total β3Gal-T5 transcripts normalized to GAPDH levels ± SEM. Black bars depict the contribution of the LTR promoter to total β3Gal-T5 transcription ± SEM. In some cases, the error bars are too small to see. Relative levels of transcripts are indicated above the bars where necessary. Assays were carried out in duplicate and repeated twice.